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For the cell apoptosis experiment, the ARP1-SRD5A1-KD and H929-SRD5A1-KD cells post 2 g/mL treatment for 48 h at a density of 2 10 5 cells were collected and stained using Annexin-V-Allophycocyanin (Annexin-V-APC) (#640941, BioLegend, San Diego, USA) and PI for 15 min in dark conditions, and the apoptosis of the cells was detected using flow cytometer

Primary antibodies diluted in 1% BSA were added dropwise to the slides and incubated overnight at 4 C

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Natural growth-hormone-releasing-hormone occurs organically in the body

A correctly reconstituted solution should be perfectly clear and free of any cloudiness, color, or floating particles