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Competent yeast cells were transformed with yeast-display plasmid pCTCON2 following the Frozen EZ yeast transformation II (Zymo Research, T2001) according to the manufacturers protocol

The National Institute of Diabetes and Digestive and Kidney Diseases lists several FDA-approved medications for chronic weight management, including orlistat, phentermine-topiramate, naltrexone-bupropion, liraglutide, semaglutide, and tirzepatide

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Treatment adherence, particularly in the early stages of T2D, is important for augmenting the effectiveness of pharmaceutical therapy and improving glycemic outcomes [32]