glutathione nitric oxide S-NITROSOGLUTATHIONE Nitric Oxide produced by a

For cell cycle analysis of cells embedded in soft agar, cells (10 5 ) were suspended in 1 mL of 0.33% noble agar, seeded in the bottom of 15-mL polypropylene tubes, cooled for 5 min on ice, and then kept at 37 C in a humidified incubator in the presence of 5% CO 2 for 24 h72 h

PMID 16254242

Hormone Mol Biol Clin Invest

For example, exogenous iron (such as ferric ammonium citrate and ferric citrate) supplementation, increased iron uptake (such as TFR1 overexpression), decreased iron storage (such as ferritin knockdown) and impaired iron efflux (such as FPN1 knockdown) contribute to iron overload and enhance the sensitivity to ferroptosis (19,20)

These iron overload-mediated double effects on lipid and iron metabolic dysregulation acts together to aggravate NASH via ferroptosis (84)