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The ripoptosome phosphorylates mixed-lineage kinase domain-like protein (MLKL), and the phosphorylated MLKL activates mitochondrial ROS production

Acknowledgements This research was supported by the Alberta Childrens Hospital Research Institute (ACHRI) and Hotchkiss Brain Institute (HBI)

NAION occurs when blood flow to the optic nerve is compromised, leading to ischemic damage

The cell pellets were thawed and lysed in a buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 10% (v/v) glycerol, 10 mM MgCl 2 , 1 mM MnCl 2 and 100 M TCEP supplemented with EDTA-free protease inhibitor cocktail (Bimake) by dounce homogenization

It is freely soluble in water at a normal pH, a property that simplifies its formulation and administration