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After stimulation, samples were lysed with RIPA and stored at 80 C

Limits of detection and quantitation (LOD and LOQ) The limits of detection and quantitation for APAP, NAPQI, and NAC were estimated employing the slope of the standard calibration curves and the residual standard deviations (Table 2)

E., and Shimada, M

also found an upregulation of the autophagy marker protein LC3 downstream of CHAC1, suggesting an autophagy-mediated induction of ferroptosis (Ying et al., 2025)