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The samples were incubated for 1 h at RT, washed three times with TBST, reacted with Supersignal West Pico solution (Pierce) for 5 min, and exposed to X-ray film (Agfa)

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The natural cycle also slows down due to environmental factors

Post-treatment, the cells were washed with 1X phosphate-buffered saline and stained with 15 M DCFDA-H2 or 5 M MitoSOX for 15 min at 37 C

Ingredients: Water, Glycerin, PEG-8, Niacinamide, Butylene Glycol, Methylpropanediol, 1,2-Hexanediol, Betaine, Glycereth-26,m Bis-PEG-18 Methyl Ether Dimethyl Silane, Polyglyceryl-10 Laurate, Bifida Ferment Lysate, Methyl Gluceth-20, Panthenol, Squalane, Glucosylrutin, Hydrogenated Lecithin, Carbomer, Caprylic/Capric Triglyceride, Tromethamine, Xanthan Gum, Propanediol, Flavor, Ethylhexylglycerin, Glutathione(500ppm), Adenosine, Ceramide NP, Sodium Phytate, Benzyl Glycol, Hydrolyzed Glycosaminoglycans, Sodium Hyaluronate, Dextrin, Theobroma Cacao (Cocoa) Extract, Beta-Glucan, Cyanocobalamin, Sodium Hyaluronate Crosspolymer, Tocopherol, Hydrolyzed Hyaluronic Acid, Hydroxypropyltrimonium Hyaluronate, Soluble Collagen, Hyaluronic Acid, Sodium Acetylated Hyaluronate, Sodium Stearoyl Glutamate