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PMID 22179958

All the experiments followed the Guidelines of Ethical Standards for Investigation of Experimental Pain in Animals

Yes with proper oversight

Vitamin D deficiency occurs when the body lacks an adequate amount of vitamin D, a vital nutrient essential for various physiological processes

The recommended B12 dosage is as follows: Men over 14 years of age should consume 2.4 mcg daily Women over 14 years of age should also consume 2.4 mcg daily Pregnant Women should consume 2.6 mcg daily Lactating Women should consume 2.8 mcg daily Be sure to discuss your interest in B12 with a medical professional prior to beginning any supplementation