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Using ImageJ software, we quantified the number of cells positive for BrdU, TUNEL or ATF4 relative to the total number of chondrocytes in the different subregions of interest or in the total growth plate

Think of this like taking a large piece of trash and breaking it into smaller, sharper shards

LC-MS untargeted metabolomics analysis For LC-MS sample analysis, the dried extracted samples were reconstituted in a mobile phase containing 50% solution (A: 0.1% formic acid in dH 2 O and (B: 0.1% formic acid in 1:1 ( v/v) MeOH and ACN)

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Post-Treatment Care: Stay hydrated