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The average firing frequency change of TH-positive ( n = 7) and TH-negative ( n = 8) neurons significantly increased during the application of 100 nM exendin-4 (Fig

In contrast, the exendin-P5 mutagenesis profile was very distinct from the other peptides with few mutations altering exendin-P5 affinity and only a very weak, albeit significant, correlation with the effect of mutations on GLP-1 in cAMP signalling assays (Figures 4-5, Supplemental Figures 9-10)