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Twenty-four hours after seeding, media was exchanged to 2 mL of DMEM +dFBS (or RPMI +dFBS /McCoys 5A +dFBS ) and 300 ng/mL of Dox (except 786-O lenti cell lines)

Increase dose by one tier every one to two weeks

doi: 10.1111/1750-3841.15447, 26

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