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supplementation can replenish depleted stores (Wang et al

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In most cases, the reason we would recommend this microdosing technique is to minimize side effects, as gastrointestinal side effects can be common with GLP-1 agonists

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On the fourth day, 1 mg ml of l-(4, 5-Dimethylthiazol-2-yl) 3, 5-diphenyIformazan reagent (MTT, Sigma- Aldrich) prepared in PBS was added to the cells and incubated for 4 h