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Western blots YR1.7 and MC38 cells were treated with 50 M statins for 18 hours, harvested by trypsinization, washed in ice-cold sterile PBS, and lysed in 1 RIPA lysis buffer (ThermoFisher, #89900) with 1 protease and phosphatase inhibitor cocktail (ThermoFisher, #78440)

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Regarding this cross-feeding mechanism, it is worth noticing a study showing that Propionibacterium acnes (now renamed Cutibacterium acnes ) was among the top 10 taxa identified by 16 S rRNA pyrosequencing in chronic rhinosinusitis (CRS) patients (Hauser et al., 2015), while another study that characterized the nasopharyngeal microbiota in healthy subjects and during rhinovirus challenge correlated the presence of Neisseria with those of Propionibacterium ( P