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RSL3 (MedChemExpress, HY-100218A) was dissolved in 10% DMSO, 40% PEG300, 5% Tween-80 and 45% saline

Promote renal calcium reabsorption Renal tubular calcium transport: upregulate the expression of renal tubular calcium channel (TRPV5), reduce urinary calcium excretion by 30% -40%, increase blood calcium concentration, and increase bone calcium deposition Evidence from preclinical studies on AOD 9604 bone protection (animal experiments) Postmenopausal osteoporosis (OVX rat model) Experimental Design: Animal: 6-month-old female SD rats underwent bilateral ovariectomy (OVX) to establish a postmenopausal osteoporosis model

May be taken with or without food

The copper-bound form is the one most in vitro studies characterise, distinct from the uncomplexed free tripeptide (CAS 49557-75-7)

We recommend refrigerating it immediately and using it with caution, and for critical applications, its safest to discard it and start with a fresh vial