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For cAMP and receptor expression assays, HEK293T cells were seeded into 6-well cell culture plates at a density of 7 10 5 cells per well

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Management: Consult prescribing physician before starting melatonin Monitor INR more frequently if using warfarin Watch for unusual bruising, bleeding Start with very low melatonin dose (0.3mg) Alternative: Work with doctor on sleep hygiene modifications before introducing supplements