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Increases in embryo-fetal resorptions and post-implantation losses were observed at 0.1 mg/kg/day in the presence of significant maternal toxicity, and fetal body weights were 7% lower than controls at 0.2 mg/kg/day

The trial reported a disease control rate (DCR) of 64.3%, with an ORR of 28.6% specifically in patients with high baseline intratumoral GSDME expression

Following treatment, cells were washed 1x in PBS before being lysed in ice-cold lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl supplemented with 1 mM EDTA, 1% Triton X-100, protease and phosphatase inhibitor cocktails), with lysates gently rocked for 15 minutes at 4C before centrifugation at 13,000 g for 5 minutes at 4C

This is not harmful accumulation but rather the intended therapeutic level

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