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Even minor bleeding inside the epidural space can complicate the procedure and increase discomfort afterward

The plastic bottom of standard 24-well plates (Corning Costar, USA) was replaced by borosilicate glass coverslips (VWR International, USA)

Unfortunately, the analogs prepared did not show biological activity

While both peptides can enhance natural growth hormone (GH) activity, their mechanisms and effects are distinct

GluK1-GluK3 are low affinity KAR subunits, due to relatively high concentration of agonist needed for their activation (K D for [ 3 H]kainate, 50100 nM) compared to GluK4 and GluK5 which are high affinity subunits (K D for [ 3 H]kainate, 5 nM) (Figure 1B)