Glutathione is a powerful antioxidant naturally present in our body
In addition, GST activities in leaves, expression of genes encoding GSTU1 and GSTF2 and GSTU1 and GSTF2 protein abundance were highest at early infection stages but decreased as visible symptoms later appeared
Figure 14a shows an increased expression (immunofluorescence) of GSTA4-4, an oxidative stress specific GST isoenzyme, in HepG2 cells after ASA treatment.NAC treatment brought the enzyme activity close to control values in 5 mol/ml ASA treated cells but not in 10 mol/ml ASA treated cells, where the enzyme expression remained significantly higher
A set of no-cell control group containing only the culture medium without cells were prepared to serve as negative control to determine background fluorescence that might be present
En el citosol, la formacin de PSSG es transitoria, excepto durante el estrs oxidativo, pudiendo estar implicada en un mecanismo de transduccin de seal en respuesta a estrs que permita su adaptacin o determine su apoptosis
