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Leukocyte-depleted parasitized blood was resuspended in an exflagellation medium (10 mM Tris, 170 mM NaCl, 10 mM glucose, 25 mM NaHCO 3 , 50 to 100 mM xanthenuric acid, and 20% fetal calf serum, pH 8.4) to 20% hematocrit and incubated for one hour at 25 C with ambient gas conditions