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Live cells were then washed with phosphate-buffered saline 1, incubated for 30 min with 5 M JC-1 (37 C, 5% CO 2 ), washed with PBS 1, trypsinized, and recovered in a final volume of 300 L of PBS

Abbreviations: CV = cardiovascular

For AsA, the supernatant was diluted by 250 mM phosphate buffer (pH 7.4) and mixed with 0.5% N-ethyl maleimide (NEM)

He added that GLP-1 therapies should form part of a structured, long-term treatment plan that includes dietary changes, lifestyle modifications and regular medical monitoring, rather than being viewed as short-term or cosmetic solutions

Diagnostic challenge: Differentiating neuropsychiatric manifestations from motor-induced cognitive slowness or medication side effects requires multidisciplinary assessment