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Investigators have also noted that the pronounced appetite-stimulating effect of MK-677 an on-target consequence of ghrelin receptor agonism in hypothalamic appetite circuits complicates body composition endpoints in metabolic studies, as caloric intake must be carefully controlled to isolate GH-axis-mediated changes from orexigenic-driven weight gain

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After 48 h of culture (at 37C in a 5% CO 2 incubator), the CCK-8 working solution was added to the sample and incubated for 1 h