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Islets were purified using an Optiprep gradient (Nycomed, Oslo, Norway) as previously described 81 and cultured for 18 h in a 37 C in a 5% CO 2 incubator in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% heat-inactivated fetal bovine serum before use in experiments

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Eli Lilly and Novo Nordisk have had multiple FDA-approved GLP-1 drugs on the market since 2022, though record-high demand, chronic supply shortages and pricing concerns have created challenges for the drugmakers