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Coverslips were washed in PBS thoroughly and blocked in PBS containing 2% BSA and 0.5% Tween-20 at RT

Ultra-structurally, the follicular cells revealed mild to moderately dilated rough endoplasmic reticulum

If the injection goes ahead, heres roughly what happens: A brief health check and consultation, a few minutes The injection itself, given into a muscle, usually the upper arm A short pause afterward to check you feel fine before you leave The whole appointment is typically done within 15 to 20 minutes

The amount of B12 that does get absorbed is directly related to the amount of intrinsic factor available