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Bile acid profiling by liquid chromatography-tandem mass spectrometry (LC-MS/MS) Liver samples (30 mg) were homogenized with 200 l pre-cooled ultrapure water, 800 L pre-cooled methanol, and 10 L internal standard, then mixed thoroughly by vortex, followed by incubation at 20 C for 20 min to allow the protein precipitation

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23 Firstly, activated Akt induces the translocation of GLUT4 vesicle from cytoplasm to membrane, promoting glucose uptake

Women of childbearing potential should be aware that tirzepatide may reduce the effectiveness of oral contraceptives, particularly during dose initiation and escalation

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