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Toxicol Pathol 41: 235-262, 2013

doi: 10.1097/MD.0000000000036730

Kugel, S

For method development and stability analysis, several crucial parameters must be optimized to ensure reliable separation: Method 0ptimization: Consistent retention and peak shape require shallow gradients, elevated column temperatures, and strong ion-pairing agents such as trifluoroacetic acid (TFA)

Common reasons for an imbalanced ratio between phosphatidylcholine and cholesterol include: Fasting Overeating High-fat diet Ketogenic diet Vegan diets Protein deficiency Low dietary choline intake Low dietary glycine intake Methylation disregulation Dirty MTHFR Dirty PEMT Dehydration Low stomach acid Ongoing intake of proton pump inhibitors (PPIs) Bacterial overgrowth of specific bacteria such as certain Clostridia species What Happens When You Dont Have Healthy Bile Composition or Bile Flow