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The screen was set up as follows, two concentrations of CHIR99021 (Stemgent - purity of 97% or less) were used: 3 and 4 M

Brown Fat and Thermogenesis Beyond white adipose tissue (the primary fat storage tissue), humans possess brown adipose tissue (BAT) specialized for thermogenesis (heat production)

We further performed doxycycline-inducible expression of oxygen-dependent NADPH oxidase (NOX) in the cytosol (TPNOX) or mitochondria (mitoTPNOX) to deplete compartmentalized NADPH, as described previously 30 , and tested the cellular sensitivity to glucose starvation (Extended Data Fig