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Antibiotic treatment 5-day old Drosophila were transferred to media containing 5% sucrose, 1% agar, 100 ug/mL ampicillin, and 50 ug/mL streptomycin (Sigma-Aldrich)

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The copper ion itself serves as a cofactor for lysyl oxidase, the enzyme responsible for collagen and elastin cross-linking, meaning GHK-Cu both stimulates production of new structural proteins and ensures they are properly stabilized

et al (2015) Oncol Lett.

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