To assess sperm motility, 5 L of sperm suspension was placed on a pre-warmed slide, and the movement of 100 spermatozoa was visually evaluated under a light microscope (CX31, Olympus, Japan) at 400 magnification
U373, U87, and HS683 cells were prepared at 2 10 5 cells per well, washed with PBS, and resuspended in 1 binding buffer
Zhu, A.J.M
Matsui M, Oshima M, Oshima H, Takaku K, Maruyama T, Yodoi J et al (1996) Early embryonic lethality caused by targeted disruption of the mouse thioredoxin gene
Intranasal delivery offers another path: a small, precise dose of glutathione nasal spray that you can use in seconds, with direct access to the rich blood supply and neural pathways inside the nose