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The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat

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The Minister reported that South Bougainville has efficiently allocated K250,000 from discretionary funds and another K250,000 from non-discretionary funds, ensuring meticulous record-keeping as a safeguard against misuse