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We present neutrophil- and NET-centered pathways as biologically plausible contributors to PCOS-associated metabolic, reproductive, and vascular dysfunction, rather than as established pathogenic drivers

After mixing, these peptides must be kept in a refrigerator and away from heat, light, and moisture to keep them stable and effective

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The clarified cell lysate was either directly submitted to further protein purification steps or flash-frozen in liquid nitrogen after addition of 10% glycerol and stored at 80 C for use in biocatalytic reactions