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Control mice received vehicle (40 mM Tris-HCl), while HFD-fed mice were randomly allocated to receive daily subcutaneous injections for 2 weeks of: vehicle, [D-Ala]-GIP (10 nM), semaglutide (10 nM), or tirzepatide (3 nM or 10 nM) ( n = 5 per group)

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