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After all of the sample molecules are eluted, a saltfree wash step is introduced to remove any hydrophobic compounds which did not elute during the elution step

One standard drink (0.6 oz ethanol): Approximately 1 hour for full metabolic clearance from peak BAC

The samples were resuspended in 1 ChIP Buffer with PIC, incubated on ice for 10 min, and then sonicated using a Bandelin ultrasonicator (Sonics, United States) set to 300 W, 50 cycles (1 s each with a duty cycle of 50%)

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Nomura T, Mochida J, Okuma M, Nishimura K, Sakabe K: Nucleus pulposus allograft retards intervertebral disc degeneration