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In addition, we employed publicly available bioinformatics annotation tools and databases, including GO Term Finder (35), STRING database (36), UniProt for researching annotations of proteins, and the Database for Annotation, Visualization and Integrated Discovery (DAVID) (37)
Repeated freeze-thaw cycling is a known nucleation trigger for amyloidogenic peptides as well as a general cause of peptide loss
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It is common to experience temporary relief immediately after the injection, but full benefits may take several days or weeks to manifest