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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

Prolotherapy has been shown to reduce pain and stiffness, and improve knee function and quality of life in knee OA patients

Retrieved from Haugan, G., & Eriksson, M