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The reaction catalyzed by -ECS/GSH1 is considered the rate-controlling step of GSH synthesis, and the activity of this enzyme is regulated by cellular levels of cysteine and glutamic acid, and feedback inhibition by -EC and GSH ( Figure 1 GSH1 (EC 6.3.2.2) is exclusively present in plastids, while GSH2 (EC 6.3.2.2) has dual localization in plastids and the cytosol, both encoded by a single gene (Wachter et al., 2005)

This mechanism may localize the effects of bioactive dopamine due to diet or local dopamine produced by peripheral tissues, and/or it may be used to inactivate dopamine when it enters circulation to prevent toxicity and catecholamine buildup (Goldstein et al., 1999)

Sci Rep 10:14426