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Sorting vector construction and gene cloning The DNA sequences encoding the GST, the N-terminal signal peptides, and the C-terminal membrane-placing module of the six GPI/TMDs (Supplementary Table S1) were synthesized and supplied in pUC57a by GENEWIZ

Postulated mechanisms of metformin action, including AMPK activation and complex I inhibition, have been demonstrated in the setting of a consistent pleiotropic activity of this drug, suggesting that several molecular targets could be involved (LaMoia and Shulman, 2021)

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IVEY, M.C

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