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HKB11 cells (wildtype and PD-1 stable pool) were cultivated in MAC1.0 v110217 medium (Gibco) with 10% heat inactivated FBS (Gibco) and, for the PD-1 overexpressing cells, 0.8 mg/mL geneticin (Invivogen) in 125 mL Optimum Growth flasks (Thomson), shaking at 120 rpm at 37 C in humidified atmosphere containing 5% CO 2

Inhibitors of glutamate release from breast cancer cells

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doi: 10.1038/s41418-017-0053-8 49 ShenXChenYTangYLuPLiuMMaoTet al

Biomarkers such as CYP370A13 expression, glutathione depletion, and oxidative stress serve as sensitive indicators of APAP exposure