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In parallel, we cloned and purified 57 Cr GPDH 705 -FLAG variant with an additional 30 amino acids from the unstructured region at the N-terminus to eliminate the possibility that the GPP domain in the construct we used in our initial cellular studies was truncated due to potentially incorrectly predicted boundaries of the chloroplast targeting sequence (CTS) ( Figure 2a , Supplementary Figure S1 , Supplementary Figure S2b , Supplementary Figure S4 )