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Reactive oxygen species (ROS) detection assay For C11-BODIPY staining, the C11-BODIPY reagent was added to the cell suspension to achieve a final concentration of 1 to 10 M, followed by incubation at 37 C for 30 to 60 min

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Springer, Berlin, pp 149158 Yu X, Pasternak T, Eiblmeier M, Ditengou F, Kochersperger P, Sun JQ, Wang H, Rennenberg H, Teale W, Paponov I, Zhou WK, Li CY, Li XG, Palme K (2013) Plastid-localized glutathione reductase2-regulated glutathione redox status is essential for Arabidopsis root apical meristem maintenance

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Merrifield had developed a method of synthesizing peptides called solid phase peptide synthesis, but other scientists told me no one had been able to use it for glucagon