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These peroxide radicals then promote the dehydrogenation of another PUFA to form phospholipid hydroperoxide (PLOOH)

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The HiLoad 16/600 Superdex 75 column (GE Healthcare) was used to separate the monomeric fractions of Nb35 with running buffer (pH 7.5) containing 20 mM HEPES and 100 mM NaCl