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Signals for protein bands were developed with Super Signal West Femto Chemiluminescent kit (Thermo Fisher Scientific, Waltham, MA) and detected by a Molecular Imager ChemiDoc XRS imaging system (Bio-Rad Laboratories, Hercules, CA)

By your mid-twenties, collagen synthesis declines at approximately 1% per year

Regular blood work to track inflammatory markers, immune cell counts, and autoimmune disease activity markers becomes even more important in this context