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The mechanism does not involve receptor downregulation or tolerance in any known way, but the signal-to-noise ratio is simply too low based on available evidence

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The multifaceted mechanism of action of 5FU involves direct incorporation of its metabolites into DNA (FdUTP) and RNA (FUTP), as well as inhibition of thymidylate synthase (TS) via FdUMP 4,5 , making it more difficult to identify rational combination partners that would enhance clinical responses without eliciting adverse systemic toxicity

Legal and Regulatory Issues: TB-500s use is not approved by regulatory bodies, and its legality may vary by region