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To investigate the effect of gene knockout on l-cysteine and GSH degradation, the engineered strains were cultured in the flasks as described above and the cells were collected by centrifugation (6000 rpm, 4 C for 5 min) after two washes with 50 mM phosphate buffer (pH7.0)

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Braun, Melsungen AG, Hesse, Germany) for all the samples

Curr Mol Pharmacol 2013

Although vitamin B12 side effects are rare, some people may experience headache, swelling, anxiousness and itching when theyve consumed too much vitamin B12 or have a reaction to a dietary supplement