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Enzymatic synthesis of t- ES amino acids To obtain (2 S ,3 S )- t- ES amino acids, a 20 ml reaction in 50 mM sodium phosphate buffer (pH 8.0) containing purified 2.5 M Cp1B or Cp2B with 5 mM ()- t -ES, 2.5 mM amino acid, 10 mM ATP and 10 mM MgCl 2 was performed at 30 C for 16 h