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Total of 20 L 10% O-pthaldialdehyde (OPA) in methanol was added to each assay mixture, vials were capped, the resulting reaction mixture incubated at 25 C for 5 min in the dark and analysed using high performance liquid chromatography (HPLC) (Merck KGaA, Darmstadt, Germany)

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Unlike fulllength hGH, AOD9604 does not meaningfully elevate IGF1 levels or worsen glucose tolerance, making its tolerability indistinguishable from placebo in human trials [2] [3]

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