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Reactions were run on a CFX384 instrument (Bio-Rad)

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Estimated dosage per spray: 1 spray 66mcg 2 sprays 132mcg 4 sprays 264mcg 8 sprays 528mcg Because nasal absorption is generally lower than injectable administration, higher overall usage amounts are commonly discussed compared with subcutaneous use

As a novel method for identifying compounds that promote the release of the physiological agonist GLP-1, we have developed and optimized a GLP-1 secretion assay

This is not because KLOW's intrinsic stability changes, but because elimination pathways become temporarily saturated