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Ice applied directly to skin can worsen irritation, so wrap ice in a thin cloth or use a gel pack

Pitolisant : Originally developed for narcolepsy, pitolisant has also been considered for PWS given its mechanism of action as a selective histamine H3 receptor antagonist/inverse agonist, which may affect wakefulness and appetite control

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The eluted protein fractions were concentrated to around 10 mL followed by dilution with salt-free AEX buffer (25 mM Tris pH 8.0, 2 mM MgCl 2 , 10 M GDP, 1 mM TCEP, and 5 % glycerol) for better binding conditions onto AEX column (Cytiva, QFF HiTrap, 2 1 mL)

Pregnancy represents an absolute contraindication for tirzepatide due to potential risks to fetal development