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Then, 5 l of laccase variants (3.4 g ml 1 of LacAnc100 and 4.0 g ml 1 of LaccID) were incubated in a 1-ml final volume containing 100 mM Britton and Robinson buffer at different pH values (2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0) in a 96-deep well plate (Sarstedt, 82.1972) covered with a lid