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Briefly, cells were lysed with NETN buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Nonidet P-40, 1 mM DTT) plus Xpert protease inhibitor cocktail solution (GeneDepot, Cat#P3100-001)

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Conclusion: Making Informed Decisions About Semaglutide Therapy Semaglutides side effect profile is well-characterized through extensive clinical trial data involving over 11,000 participants in the SUSTAIN and PIONEER programs, thousands more in the STEP weight management trials, and ongoing real-world surveillance

Neuron 18 , 483491 (1997)