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In short, the samples were put in ice-cold PBS and centrifuged for 20 s at 100 g at room temperature followed by washing three times with 37 C PBS and by digestion in trypsin and DNase at 37 C for 10 min
A synergistic action in which glucagon receptor activation enhances energy expenditure whilst GLP-1 receptor agonists reduce calorie intake and both GLP-1/GIP potentiate the incretin effect to modulate the diabetogenic effect of glucagon agonists
