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The cost of a replacement vial is always less than the cost of running an unreliable protocol

In brief, HUVECs were lysed in the Co-IP buffer containing 20 mM Tris-HCl (pH7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 0.5% protease inhibitor cocktail on ice for 20 min

Meanwhile, pPICZA-CBBBP, and pPICZA-DSIP plasmids were also constructed by using the same way

This step is very important for the right dose